The Edinburgh Protein Production Facility (EPPF)

 
 

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Rapid solutions to the production of proteins and the biophysical chracterisation of their ligands underpins many of the important questions being asked in structural and translational biology today. The Wellcome Trust and University of Edinburgh funded Protein Production Facility (EPPF) provides researchers with access to state-of-the-art equipment and excellent end-user core facilities to address these questions.


The EPPF labs are located on the 3rd floor of the Michael Swann building  in custom renovated labs, and consist of several specialized areas. The EPPF has just upgraded its LC equipment. The purification suite consists of four ÄKTA Pure 25 systems and two ÄKTAXpress systems. ÄKTA Pure 25 is a new generation, flexible and intuitive chromatography systems, running UNICORN 7.1 control software, designed for fast purification of proteins, peptides, and nucleic acids from microgram to gram levels.

Other resources include a cell culture suite and biophysical characterization labs with an  extensive set of analysis and structural determination platforms, including SPR BIAcore T200, Auto-iTC200, CD, DLS, SEC-MALS, multimode spectroscopic platforms.

The EPPF aims to offer the scientific community of the University, and beyond, a comprehensive and world class end user facility, with services ranging from a complete service to any of the many selected and specialized steps involved in the production and analysis of proteins and their ligands.

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Staff and Training:

In addition to the wide range of high quality instrumentation incorporated in the facility, the EPPF also provides users access to the expertise (in excess of 85 combined years) of the highly trained facility staff. The EPPF staff are responsible for the management of the laboratory and have a wide experience in molecular biology, protein expression and purification and biophysical analysis of proteins and their ligands. EPPF staff will provide fully assisted technical support and training for the infrequent user. The EPPF is also an excellent equipment resource for the experienced user. Practical training (basic and advanced) for users, ranging from individually tailored one-on-one instruction to frequent centralized training courses, is also provided by the EPPF. Click here for more information or below for enquiries.

Enquiries: Dr. Martin Wear

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Costs/access charges for use of EPPF:

The EPPF operates primarily in ‘hotel model’ whereby end-users book in and use well maintained equipment under the guidance of the EPPF staff. The access charging is tiered depending on multiple factors, including EPPF-staff involvement, maintenance  recovery, consumable usage, user funding body and whether they are internal or external, academic or industrial.

Please click here for individual platform/resource costings.

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Full Service Work:

If you do not have the time to undertake the experiments yourself or do not intend to use the resources and equipment that frequently, there may be an opportunity for the EPPF to provide a full service where our experienced staff will undertake the experiments for you. In many cases this can be most beneficial for forwarding the project as the staff are both efficient and have experience to optimise the conditions and exploit other techniques that less experienced users may not have been aware of. Please be aware however, that there will be additional costs involved and mutually agreeable time-scales and milestones involved in engaging the EPPF in full-service work.


Enquiries: Dr. Martin Wear

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Commercialisation and consultancy:

Opportunities exist for engagement of the EPPF for commercialisation, consultancy and service. In bridging biology, chemistry and medicine EPPF is uniquely placed to offer services to academics, SME's and Pharma ranging from HT-protein production and ligand characterisation through to pre-clinical drug discovery.

 

Enquiries: Contact Dr. Martin Wear or Dr. Keith Finlayson to discuss commercial opportunities with EPPF or visit Edinburgh Research and Innovation (www.research-innovation.ed.ac.uk) for all aspects of knowledge transfer, licensing, services and consultancy at the University of Edinburgh.

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contacts:


Facilities Manager:

Dr. Martin Wear

Rm. Swann 3.20

Tel (+44) 0131 6507054

Fax (+44) 0131 6507055

email: martin.wear@ed.ac.uk


Lab Manager:

Mrs. Sandra Bruce

Rm. Swann 3.19

Tel (+44) 0131 6507054

Fax (+44) 0131 6507055

email: sabruce@staffmail.ed.ac.uk


Protein Technologist:

Dr. Liz Blackburn

Rm. Swann 3.19

Tel (+44) 0131 6507054

Fax (+44) 0131 6507055

email: E.A.Blackburn@ed.ac.uk


Protein Technologist:

Dr. Matt Nowicki

Rm. Swann 3.19

Tel (+44) 0131 6507054

Fax (+44) 0131 6507055

email: matthew.nowicki@ed.ac.uk


Facilities:


CTCB-Home

Protein Production Biophysical Characterisation

In Silico Screening

Training


Links:


On-line Booking

Core Column Library

EPPF User Group


Access Charges/Costs:


Access Charges/Costs

 

Location:

The University of Edinburgh,

Level 3 Michael Swann Building,

King’s Buildings,

Mayfield Rd.,

EH9 3JR,

UK


Labs 3.5 - 3.10 & 3.14 - 3.17

Core Facilities for Protein Production and Characterisation